Abstract
AbstractHelicobacter pyloriis closely connected to upper gastrointestinal tract diseases including gastric cancer. Transgenic plants are found to be successful in expressing the bacterial antigens, which could elicit an immune response when consumed. The Cytotoxicity-associated immunodominant antigen protein (cagA) ofH. pyloriis kindred with pathogenicity and cancer risk. We expressed thecagAtransgenically in the brinjal. We amplified thecagAgene fromH. pyloristrain 26695 chromosomal DNA and transformed it into brinjal callus derived from leaf explants using the pBI121 expression vector. The stable expression and accumulation of the recombinantcagAgene were confirmed by using quantitative real-time PCR, western blot analysis and ELISA. The RT-PCR, western blot and ELISA showed stable expression ofcagAgene in the transgenic lines B3, B5, B11, B17 and B21. Among them, B11 and B17 samples showed higher expression of thecagAcompared to the other samples. Besides, the immunohistochemistry assay showed the abundant expression ofcagAprotein in the parenchymal regions of the transgenic plants. Out of the 52 plants, a set five plants were found to be positive forcagAexpression. Our experimental outcomes can be used further to design the vaccines againstH. pylorifrom the transgenic brinjal plants.
Publisher
Cold Spring Harbor Laboratory
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