DNA replication is not a limiting factor for rapid growth ofVibrio natriegens

Abstract

AbstractDNA replication prior to cell division is essential for the proliferation of all cells. Bacterial chromosomes are replicated bidirectionally from a single origin of replication, with replication proceeding at about 1000 bp per second. For the best-studied model organism,Escherichia coli, this translates into a replication time of about 40 min for its 4.6 Mb chromosome. Nevertheless,E. colican propagate by overlapping replication cycles with a maximum short doubling time of 20 min. The fastest growing bacterium known today,Vibrio natriegens, is able to replicate with a generation time of less than 10 min. It has a bipartite genome with chromosome sizes of 3.2 and 1.9 Mb. Is simultaneous replication from two origins a prerequisite for its rapid growth? We fused the two chromosomes ofV. natriegensto create a strain carrying a 5.2 Mb chromosome with a single origin of replication. Compared to the wild-type, this strain showed little deviation in growth rate. This suggests that the split genome is not a prerequisite for rapid growth, and that DNA replication is not an important growth rate-limiting factor.