Antimitotic Activity ofSamanea samanLeaf Extract in the In Vitro Development ofTripneustes gratillaEmbryo

Author:

Lam Justin Riley Y.ORCID,Casimpan Guinevere A.,Batoy Christine May L.,Cebedo Ma. Felaine,Cuesta Jann Ycleo T.,Gervacio Jecell,Grapa Joshua Benjamin R.,Mabelin Ma. Katrina Ada F.,Pepito Merry Grace S.,Rama Justine Mae A.,Simporios Daniel Joseph Z.,Villanueva Zackaree Michael A.,Villaruel Jolienne Abbygail M.,Gwendolyn D. Pepito,Adrian Christopher A. Lu

Abstract

ABSTRACTTITLEAntimitotic Activity ofSamanea samanLeaf Extract in the In Vitro Development ofTripneustes gratillaEmbryoINTRODUCTIONCommercially available anticancer drugs are expensive and may have side effects. This led the researchers to look into alternative plant sources. One of these is acacia orS. saman.OBJECTIVETo determine whetherS. samanleaf extract possesses antimitotic activity onT. gratillaembryos using vincristine sulfate as positive control.METHODOLOGYS. samanleaves were extracted using hexane, CCl4, CHCl3, and water.T. gratillaembryos were fertilized in vitro. The fractions, vincristine sulfate, and DMSO in filtered sea water were added with the fertilized embryos in petri dishes. Samples were taken at 15 minutes after fertilization and every 30 minutes thereafter until the negative control reached a 32-cell stage. Fifty cells and their mean cell stage was evaluated per treatment. One-way ANOVA and Tukey tests were performed.RESULTSSignificant differences were seen starting at 75 minutes post fertilization up until the 165thminute using One-way ANOVA. The Tukey test showed that all aqueous and CCl4as well as hexane (200, 400, 800 ppm) extracts had no significant difference compared to vincristine sulfate; while all CHCl3, hexane (100 ppm), and CCl4(100 ppm) extracts showed a significant difference compared to vincristineCONCLUSIONThe aqueous, hexane, and carbon tetrachloride extracts possess potential antimitotic activity onT. gratillaembryos. Thus, it is a potential alternative to vincristine sulfate as an antimitotic agent.

Publisher

Cold Spring Harbor Laboratory

Reference47 articles.

1. National Cancer Institute. What Is Cancer? [Internet]. National Cancer Institute. 2018 [cited 18 Nov 2018]. Available from: https://www.cancer.gov/about-cancer/understanding/what-is-cancer

2. Influence of lifestyle factors on breast cancer risk;Breast Care [Internet,2014

3. International Agency for Research on Cancer. Latest global cancer data: cancer burden rises to 18.1 million new cases and 9.6 million cancer deaths in 2018 [Internet]. Geneva: World Health Organization; 2018 Sep 12 [cited 2018 Nov 18]. Available from: https://www.who.int/cancer/PRGlobocanFinal.pdf

4. Bersales L. Deaths in the Philippines, 2016 [Internet]. Philippines: Philippine Statistics Authority; 2018 Feb 12 [cited 2018 Nov 18]. Available from: https://psa.gov.ph/content/deaths-philippines-2016?fbclid=IwAR19qAnMma01gyh70PmB5ejbETKiVLym11NupeT-1cTUqrfpml_JdtD9xaw

5. Abbas Z , Rehman S. An overview of cancer treatment modalities [Internet]. IntechOpen. 2018 Sep 19

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