Abstract
Abstract SummaryBrain extracellular matrix (ECM) structure mediates many aspects of neuronal function. Probing changes in ECM structure could provide insights into aging and neurological disease. Herein, we demonstrate the ability to characterize changes in brain ECM structure using multiple particle tracking (MPT). MPT was carried out in organotypic rat brain slices to detect induced and naturally occurring changes in ECM structure. Induced degradation of neural ECM led to a significant increase in nanoparticle diffusive ability in the brain extracellular space. For structural changes that occur naturally during development, an inverse relationship existed between age and nanoparticle diffusion. Using the age-dependent dataset, we applied extreme gradient boosting (XGBoost) to generate models capable of classifying nanoparticle trajectories. Collectively, this work demonstrates the utility of MPT combined with machine learning for measuring changes in brain ECM structure and predicting associated complex features such as developmental age.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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