Detection of the altered metabolites of Raji cells in the presence of Epstein-Barr virus (EBV) using 1HNMR Spectroscopy

Abstract

AbstractEBV is one of the most common viruses in humans and is directly implicated in carcinogenesis. The present study aimed to detect the altered metabolites of Raji cells in the presence of Epstein-Barr virus (EBV) using 1H NMR by NOESY technique. The cells and EBV were maintained in RPMI 1640 and 1 ml of cells were transfected into falcon tubes containing cells and were incubated at 37°C for 2 hours. Metabolites extracted using water or chloroform/methanol, lyophilized and sent for 1H NMR analysis by NOESY technique. The NMR spectrum comprising of Fourier Transformed information about metabolites in the control and treated were imported into MATLAB (v.7.8.0.347) software and the metabolic cycles were determined using Metabo-Analyst software. The data have demonstrated that infected cells leads to proliferation and subsequent immortalization of cell lines through cellular replication machinery recruitment and changes the metabolic profile and promotes vital metabolites such as the carbohydrates engage in pentose phosphate and glycolactic, biosynthesis of nucleotide and amino acid pathways. The results also indicate that essential amino acids are required for protecting viral structure and the function of viral genes. Therefore, EBV infection of cells leads to the sustained elevation of cell growth and cell immortalization.