Author:
Wudzinska Aleksandra,Mitchell Leslie A.,Brosh Ran,Bosco Nazario,Boccalatte Francesco,Aifantis Ioannis,Kim Sang Y.,Davoli Teresa,Boeke Jef D.
Abstract
AbstractAlthough the structure and function of the alphoid-tetO Human Artificial Chromosome (tetO-HAC) has been previously described in cell culture models and somatically in the mouse, in vivo persistence and stability throughout meiosis and across generations were not evaluated. Here we report germline transmission of a circular tetO-HAC across three mouse generations without observable health or reproductive deficiencies. Furthermore, we show that the tetO-HAC is maintained without selection as an episome and can be efficiently transmitted by both ova and sperm.
Publisher
Cold Spring Harbor Laboratory