Abstract
AbstractWe here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5Å ribosome averages from yeast.
Publisher
Cold Spring Harbor Laboratory
Reference33 articles.
1. Exploring high-resolution cryo-ET and subtomogram averaging capabilities of contemporary DEDs;J. Struct. Biol,2022
2. New tools for automated cryo-EM single-particle analysis in RELION-4.0
3. Single-particle cryo-EM at atomic resolution;Nat. 2020 5877832,2020
4. Cryo-Electron Tomography and Subtomogram Averaging
5. Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 □Å in cells;Nat. Methods 2021 182,2021
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献