Author:
Suárez Vanessa Tardillo,Karepina Elizaveta,Chevallet Mireille,Gallet Benoit,Cottet-Rousselle Cécile,Charbonnier Peggy,Moriscot Christine,Michaud-Soret Isabelle,Bal Wojciech,Fuchs Alexandra,Tucoulou Rémi,Jouneau Pierre-Henri,Veronesi Giulia,Deniaud Aurélien
Abstract
AbstractThe impact on human health of the increasing use of silver nanoparticles (AgNPs) in medical devices remains understudied, even though AgNP-containing dressings are known to release silver in the bloodstream leading to accumulation and slow clearance in the liver. Cellular studies have shown the intracellular dissolution of AgNPs within endo-lysosomes followed by Ag(I) binding to biomolecular thiolate-containing molecules. However, the precise subcellular distribution of Ag(I) and the nature of the disrupted physiological pathways remained unknown. Novel imaging approaches enabled us to visualize the trafficking of AgNP-containing lysosomes towards a perinuclear location and a direct nuclear transfer of Ag(I) species with accumulation in the nucleoli. These Ag(I) species impaired nuclear receptor activity, disrupting critical mechanisms of liver physiology in very low dose exposure scenarios, thus justifying further research into defining a framework for the safe use of AgNPs.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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