CHARACTERISATION OF FUNGI OF STORED COMMON BEAN CULTIVARS GROWN IN MENOUA DIVISION, CAMEROON

Abstract

AbstractCommon bean is a legume grown globally especially in developing countries including Cameroon for human consumption. In Cameroon it is grown in a wide variety of agro ecological zones in quantities enough to last through the off growing season after harvest. Stored common bean after harvest in Cameroon are prone to fungal spoilage which contributes to post harvest losses. This study aimed at characterising storage fungi on cultivars of stored common bean using morphological and molecular techniques. Fungi were isolated from six stored cultivars of common bean; Kidney bean, Black bean, Navy bean, Pinto bean, Pea bean and Large seeded bean plated on potato dextrose agar media. Cultural, micro-morphological and molecular techniques were used to characterise the isolates. A total of four fungi isolates resulted. Homology matches of the gene sequences in the Genbank databases identified the isolates to be; Xylaria hypoxylon, Aspergillus flavus, Penicillium aethiopicum and Fusarium oxysporium. Phylogenetic analysis and multiple sequence alignment showed these isolates were of distinct species. The species of fungi recovered from the stored cultivars signified poor preservation methods carried out after harvest. Effective management and control of these fungal species in common bean at storage will help reduce post-harvest losses and increase seed health.