Cyniclomyces guttulatusis an opportunistic pathogen in rabbits with coccidiosis

Abstract

AbstractCyniclomyces guttulatusis a common inhabitant of the gastrointestinal tract in rabbits, and large numbers are often present in feces of diarrheic rabbits. However, its relation with rabbit diarrhea has not been clearly identified. We isolated aC. guttulatusZhejiang strain from a rabbit with severe diarrhea and optimized the culture conditions in YPG medium. The sequenced 18S and 26S ribosomal DNA fragments were 1559bp and 632bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from the dog and 100% homology with the 26S ribosomal sequence of the DPA-CGR1 and CGDPA-GP1 isolates from the rabbit and guinea pig. Our isolate was not pathogenic to healthy SPF rabbits. Instead, rabbits inoculated with the yeast had a slightly better body weight gain and higher food intake. Rabbits co-inoculated withC. guttulatusand the coccidian,E. intestinalisdeveloped more severe coccidiosis as shown by clinical signs, and decreased body weight gain, diarrhea and death, associated with significantly higher fecal output ofC. guttulatusvegetative cells but lower coccidian oocysts output than the rabbits inoculated withC. guttulatusorE. intestinalisalone. We also surveyed the prevalence ofC. guttulatusin rabbits and found a positive rate of 83% in Zhejiang province. Our results indicate thatC. guttulatusalone is not pathogenic to healthy rabbits, but could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.Author summaryCyniclomyces guttulatus, a commensal yeast in rabbit gastrointestinal tract, is very commonly seen in diarrhea cases. However, it is unclear whether it causes or is a co-cause of diarrhea with other pathogens. Here, aC. guttulatusZhejiang strain was firstly isolated from a rabbit with severe diarrhea and the culture conditions in YPG medium were optimized. Then, it was identified in morphology and molecular. It was agreed with the previous description in morphology and showed a closer phylogenetic relationship with other strains originated from herbivores than those from the carnivore. Finally, theC. guttulatusZhejiang strain was inoculated to SPF rabbits singly or co-inoculated withEimeria intestinalis. All of the results in animal assays showC. guttulatusalone is not pathogenic but seems a probiotic microorganism in rabbits. However, it could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.