Author:
Plessy Charles,Pascarella Giovanni,Bertin Nicolas,Akalin Altuna,Carrieri Claudia,Vassalli Anne,Lazarevic Dejan,Severin Jessica,Vlachouli Christina,Simone Roberto,Faulkner Geoffrey J.,Kawai Jun,Daub Carsten O.,Zucchelli Silvia,Hayashizaki Yoshihide,Mombaerts Peter,Lenhard Boris,Gustincich Stefano,Carninci Piero
Abstract
Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR geneOlfr160(M72) can drive OSN-specific expression in transgenic mice.
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics (clinical),Genetics
Cited by
53 articles.
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