Linear and Neural Network Models for Predicting N-glycosylation in Chinese Hamster Ovary Cells Based on B4GALT Levels

Abstract

AbstractGlycosylation is an essential modification to proteins that has positive effects, such as improving the half-life of antibodies, and negative effects, such as promoting cancers. Despite the importance of glycosylation, predictive models have been lacking. This article constructs linear and neural network models for the prediction of the distribution of glycans on N-glycosylation sites. The models are trained on data containing normalized B4GALT levels in Chinese Hamster Ovary cells. The ANN models achieve a median prediction error of 1.39%, which is 10-fold smaller than for previously published models, and a narrow error distribution. We also discuss issues with other models reported in the literature. We provide all of the software used in this work, allowing other researchers to reproduce the work and reuse or improve the code in future endeavors.