Abstract
AbstractDeciphering the connectome, the ensemble of synaptic connections that underlie brain function, is a central goal of neuroscience research. Here, we report mapping of connections between presynaptic and postsynaptic partners in a living vertebrate nervous system, that of the zebrafish, through the successful adaptation of thetrans-Tango genetic approach, first developed for anterograde transsynaptic tracing inDrosophila. Neural connections were visualized between synaptic partners in the larval retina and brain and followed over development. Results were corroborated by functional experiments in which optogenetic activation of retinal ganglion cells elicited responses in neurons of the optic tectum, as measured bytrans-Tango-dependent expression of a genetically encoded calcium indicator.Transsynaptic signaling throughtrans-Tango reveals predicted as well as previously undescribed synaptic connections in the zebrafish brain, providing a valuablein vivotool to monitor and interrogate neural circuits over time.
Publisher
Cold Spring Harbor Laboratory
Cited by
4 articles.
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