Author:
Lone Bashir Ahmad,Tabassum Misbah,Bhushan Anil,Dhiman Urvashi,Rani Dixhya,Gupta Prem N.,Mondhe D. M.,Gairola Sumeet,Gupta Prasoon
Abstract
AbstractTwo novel steroidal saponins, Trilliumosides A (1) and B (2) were isolated from the rhizomes ofTrillium govanianumby bioactivity-guided phytochemical investigation along with seven known compounds protodioscin (3) govanoside B (4), borassoside E (5), 20-hydroxyecdysone (6), 5-20-hydroxyecdysone (7), govanic acid (8), and diosgenin (9). The structure of novel compounds 1-2 were established using spectroscopic methods such as 1D, 2D NMR data and HR-ESI-MS. The isolated compounds were evaluated forin-vitrocytotoxic activity against a panel of human cancer cell lines. Compound1showed significant cytotoxic activity against A-549 (Lung) and SW-620 (Colon) cell lines with IC50values of 1.83 and 1.85 µM, whereas compound (2) IC50value against A-549 cell line was found to be 1.79 µM. Among previously known compounds (3), (5) and (9) their cytotoxic IC50value was found to be in the range of 5-10 µM. In detailed anticancer analysis compound (2) was seen inhibiting colony forming potential andin-vitromigration in the A-549 cell line. Furthermore, the mechanistic study of compound (2) on the A-549 cell line revealed characteristic changes including nuclear morphology, increased ROS generation, and reduced levels of MMP. Above mentioned events eventually induce apoptosis, a key hallmark in cancer studies, by upregulating the pro-apoptotic protein BAX and downregulating the anti-apoptotic protein BCL-2 thereby activating Caspase-3. Our study reports the first mechanistic anticancer evaluation of the compounds isolated from the rhizomes ofTrillium govanianumwith remarkable activity in the desired micro molar range.Graphical abstract
Publisher
Cold Spring Harbor Laboratory
Reference35 articles.
1. Review on Ethnomedicinal Plant: Trillium govanianum Wall;Ex D. Don. Int. J. Theor. Appl. Sci,2019
2. Life-history monographs of Japanese plants. 2: Trillium camschatcense;Ker-Gawl, Plant Species Biol,2005
3. Trillium-toward sustainable utilization of a biologically distinct genus valued for traditional medicine;Bot. Rev,2019
4. Diversity distribution and indigenous uses of medicinal plants in Parbati valley of Kullu district in Himachal Pradesh, North-western Himalaya;Asian. J. Adv. Basic Sci,2014
5. Ethnobotanical observations in the Mornaula reserve forest of Komoun, West Himalaya;India. Ethnobot. Leafl,2010
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献