Abstract
ABSTRACTBackgroundTraditional laboratory-based surveillance of gastrointestinal pathogens is time-consuming, which can impact successful outbreak detection. The INTEGRATE study investigated the utility of metagenomic and metatranscriptomic sequencing for the rapid diagnosis of community-associated gastrointestinal infections.MethodsWe performed an observational study using stool samples from 1,407 patients with acute gastroenteritis, recruited via general practitioners in the UK. 1,067 stool samples were processed using i) routine clinical methods, ii) a molecular multiplex real-time polymerase chain reaction (PCR) assay, and iii) DNA and RNA sequencing. The relationship between assigned taxonomy, routine clinical diagnostics, and PCR was determined with multivariable linear regression models.FindingsThere is a strong, positive relationship between the identification of pathogens in metatranscriptomic reads, and positive results from traditional diagnostics for five out of fifteen pathogens:Campylobacter(p<0·001),Cryptosporidium(p<0·001),Salmonella(p<0·01),Rotavirus(p<0·001) andSapovirus(p<0·001). Metagenomic sequencing displayed this relationship for two out of fifteen pathogens:Campylobacter(p<0·001) andSalmonella(p<0·01).Strong positive relationships between metatranscriptomic reads and positive PCR results were observed for six out of fourteen pathogens: Adenovirus,Campylobacter, Cryptosporidium, Norovirus, RotavirusandSapovirus(p<0·001). In metagenomic data, the same relationship was observed for four out of fourteen pathogens: Adenovirus (p<0·001),Campylobacter(p<0·001),Salmonella(p<0·05) andShigella(p<0·01).A comprehensive transcriptomic profile ofSalmonellaEnteritidis was recovered from the stool of a patient with a subsequently confirmedSalmonellainfection.InterpretationThe metatranscriptomic strategy successfully detected a variety of gastrointestinal pathogens and provided viral and bacterial gene expression profiling directly from stool. We propose that metatranscriptomics could be considered for the future surveillance of gastrointestinal pathogens.FundingThis publication presents independent research supported by the Health Innovation Challenge Fund (WT096200, HICF-T5-354), a parallel funding partnership between the Department of Health and Wellcome Trust. The views expressed in this publication are those of the author(s) and not necessarily those of the Department of Health or Wellcome Trust.
Publisher
Cold Spring Harbor Laboratory