Abstract
AbstractSpermatogenesis leads to the formation of functional sperm cells. Here we have applied high-pressure freezing in combination with transmission electron microscopy (TEM) to study the ultrastructure of sperm development in subadult males of the praying mantidHierodula membranacea. We show the ultrastructure of different stages of sperm development in this species. In addition, we have applied serial-section electron tomography of the nebenkern to demonstrate in three dimensions (3D) that this organelle is composed of two interwoven segments that are connected by a zipper-like structure at opposing positions. Our approach will enable further ultrastructural analyses of the nebenkern also in other organisms.
Publisher
Cold Spring Harbor Laboratory
Reference46 articles.
1. Primi stadi embrionali della Mantis religiosa;Monit Zool Ital,1897
2. The evolution of the sex chromosomes I. The XO and X1X2Y mechanisms in praying mantids;J Genet,1941
3. POLARIZATION, KINETOCHORE MOVEMENTS, AND BIVALENT STRUCTURE IN THE MEIOSIS OF MALE MANTIDS
4. The chromosomes of mantids (Orthoptera: manteidae) in relation to taxonomy;Chromosoma,1950
5. Evolution and the meaning of metaphase;J Cell Sci,1992