Mycobacterium tuberculosisemploys atypical and different classes of B12switches to control separate operons

Abstract

AbstractVitamin B12(B12), an essential cofactor in all domains of life, is producedde novoby only a small subset of prokaryotes, but B12-sensing riboswitches are some of the most widely distributed riboswitches in bacteria.Mycobacterium tuberculosis, the causative agent of the ongoing tuberculosis pandemic, encodes two distinct vitamin B12riboswitches. One controls the expression ofmetE, encoding a B12-independent methionine synthase, while the other is located upstream ofppe2,a PE/PPE family gene whose function is still unresolved. Here, we analyse ligand sensing, secondary structure architecture, and gene expression control mechanisms of these two riboswitches. Our results provide the first evidence of direct ligand binding bymetEandppe2riboswitches and show that the two switches exhibit different preferences for natural isoforms of B12, use distinct regulatory and structural elements, and act as translational OFF switches. Based on our results, we propose that theppe2switch represents a new Class IIc of B12-sensing riboswitches. Moreover, we have identified small translated open reading frames (uORFs) upstream of bothmetEandppe2, which modulate the expression of the respective downstream genes in opposite directions. Translation of themetEriboswitch uORF suppresses MetE expression, while translation of the uORF in theppe2switch is essential for PPE2 expression via the synthesis of a uORF-PPE2 fusion protein. In summary, our findings reveal an unexpected diversity and complexity of B12-dependentcis-regulation inM. tuberculosis, with potential implications for host-pathogen interactions.