Evolution of miR-155 and its probable targets:MST1R, Adam10, andCD9genes expression levels in adults and children gastritis patients withH. pyloriinfection

Abstract

AbstractIntroductionH. pyloriaccounts for the main factor of gastric cancer which contributes to an immune response with the promotion of inflammation. Recently, the effect of miRNAs on the prognosis of diseases is gaining the attraction of investigators. Herein, we studied the expression levels of miR-155 and its identified targets (MST1R, Adam10, andCD9) along with miR-155 expression correlation with virulence factors ofH. pylori(vacAandcag genes),H. pyloricolonization, and inflammation, in patients’ candidates for gastric endoscopy due to gastritis.Methodsin this study total of 50 and 26 biopsy samples were taken from adults and children respectively. Biochemical and molecular identification of samples was performed using culture and PCR ofureC, 16sRNA, along with amplification ofvacAandcagAgenes for pathogenicity of bacteria. The qRT-PCR was carried out using STEM-LOOP RT-PCR (dye-based) for the evaluation of miR-155 expression level andAdam10, CD9, andMST1Rexpression levels. All Real-Time PCR reactions were carried out in triplicate and data analysis was conducted using REST.ResultsA total of 30 out of 17 biopsy samples in adults and children were positive forH. pyloriin both PCR and culture, respectively. The expression level of miR-155 is closely related to theH. pylori infectionand the down-regulation ofCD9, andMST1Rgenes inH. pylori(+) samples compared toH. pylori(-) in adults’ biopsy (p=0.0001). Although, there wasn’t any relation betweencagAandvacAgenes with the expression of miR-155 in evaluated biopsy samples in both adults and children.iConclusionThis study for the first time revealed that the expression ofMST1R, CD9, andadam10 geneswas relatively related to the expression of miR-155, and indicated that the miR-155 overexpression promoted the poor prognosis ofH. pyloriinfection in adults.