Author:
H-Vazquez Juan,Cal-Sabater Paloma,Arribas-Rodríguez Elisa,Fiz-López Aida,Perez-Segurado Candido,Martín-Muñoz Álvaro,Prado Ángel De,de la Fuente Graciani Ignacio,González Sonia Pérez,Gutiérrez Sara,Tellería Pablo,Novoa Cristina,Rello Silvia Rojo,Garcia-Blesa Antonio,Sedano Rosa,Martínez García Ana María,Pérez Sonsoles Garcinuño,Domínguez-Gil Marta,García Cristina Hernán,Guerra Mª Mercedes,Muñoz-Sánchez Eduardo,Barragan-Pérez Cristina,Morales Soraya Diez,Donnarumma Oriana Casazza,Pollo Daniel Ramos,Solla Natalia Santamarta,Álvarez Manzanares Paula Mª,Bravo Sara,Alonso Cristina García,Nieto Ángel Tesedo,López Moreno Elisabet Carmen,Cabrera Sanz María Esther,Olmedo Sara Borge,de Paula Ortiz Miguel,Asenjo Alberto Castellanos,Alonso Jenifer Gay,Garrote José A.,Arranz Eduardo,Eiros José María,Santiago Fernando Rescalvo,Villegas Carolina Quevedo,Tamayo Eduardo,Orduña Antonio,Dueñas Carlos,Peñarrubia María Jesús,Cuesta-Sancho Sara,Montoya María,Bernardo David
Abstract
ABSTRACTCOVID-19 affects the population unequally with a higher impact on aged and immunosuppressed people. Hence, we assessed the effect of SARS-CoV-2 vaccination in immune compromised patients (older adults and oncohematologic patients), compared with healthy counterparts. While the acquired humoral and cellular memory did not predict subsequent infection 18 months after full immunization, spectral and computational cytometry revealed several subsets within the CD8+T-cells, B-cells, NK cells, monocytes and CD45RA+CCR7-Tγδ cells differentially expressed in further infected and non-infected individuals not just following immunization, but also prior to that. Of note, up to 7 subsets were found within the CD45RA+CCR7-Tγδ population with some of them being expanded and other decreased in subsequently infected individuals. Moreover, some of these subsets also predicted COVID-induced hospitalization in oncohematologic patients. Therefore, we hereby have identified several cellular subsets that, even before vaccination, strongly related to COVID-19 vulnerability as opposed to the acquisition of cellular and/or humoral memory following vaccination with SARS-CoV- 2 mRNA vaccines.SUMMARYAn in depth and unbiased spectral cytometry characterization of the immune system before and after COVID-19 vaccination can predict not just subsequent PCR-confirmed infection, but also COVID-induced hospitalization in immune compromised patients.
Publisher
Cold Spring Harbor Laboratory