Abstract
AbstractThe potential of mesenchymal stem cells (MSCs) for tissue repair and regeneration has garnered great attention. While MSC interaction with microbes at sites of tissue damage and inflammation is likely, especially in the gut, the consequences of bacterial association have yet to be elucidated. This study investigated the effect ofSalmonella entericasspentericaserotype Typhimurium on MSC trilineage differentiation path and mechanism. Through examination of key markers of differentiation, immunomodulatory regulators, and inflammatory cytokines, we demonstrated thatSalmonellaaltered osteogenic and chondrogenic differentiation pathways in human and goat adipose-derived MSCs. Gene expression profiles defined signaling pathway alterations in response toSalmonellaassociation not observed in epithelial cells. We uncovered significant differential expression (P< 0.05) of genes associated with anti-apoptotic and pro-proliferative responses in MSCs duringSalmonellachallenge. These observations led us to conclude that bacteria, specificallySalmonella,induce pathways that influence functional differentiation trajectories in MSCs, thus implicating substantial microbial influence on MSC physiology and immune activity.
Publisher
Cold Spring Harbor Laboratory