Lipid fingerprints are similar between SLC6 transporters in the neuronal membrane

Abstract

ABSTRACTWe use molecular dynamics simulations to characterise the local lipid annulus, or “fingerprint”, of three SLC6 transporters (dDAT, hSERT, and GlyT2) embedded into a complex neuronal membrane. New membrane analysis tools were created to improve leaflet detection and leaflet-dependent properties. Overall, lipid fingerprints are comprised of similar lipids when grouped by headgroup or tail saturation. The enrichment and depletion of specific lipids, including sites of cholesterol contacts, varies between transporters. The subtle differences in lipid fingerprints results in varying membrane biophysical properties near the transporter. Through comparisons to previous literature, we highlight that the lipid-fingerprint in complex membranes is highly dependent on membrane composition. Furthermore, through embedding these transporters in a simplified model membrane, we show that the simplified membrane is not able to capture the biophysical properties of the complex membrane. Our results further characterise how the presence and identity of membrane proteins affects the complex interplay of lipid-protein interactions, including the local lipid environment and membrane biophysical properties.HIGHLIGHTSLipid fingerprints are comprised of similar lipid classesSites of specific lipid contacts, including CHOL, varies between transportersChanges in lipid annulus result in variable local membrane biophysical propertiesMembrane composition, including that of complex membranes, affects lipid annulusGRAPHICAL ABSTRACT