Novel method for the extraction of DNA from Lamniformes tooth and denticle enamel Suitable for PCR

Author:

Swig BryanORCID,Collier Ralph S.

Abstract

AbstractExtraction of high-quality genomic DNA from Lamniformes tooth fragment and dermal denticle enamel is discussed as a method of identifying individual sharks. We describe a procedure that permits isolation of genomic DNA of satisfactory size and quality for PCR analysis, as well as for most routine cloning applications. This method should allow for the non-invasive collection of genomic samples from Lamniformes.

Publisher

Cold Spring Harbor Laboratory

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