Abstract
AbstractWe describe our initial studies in the development of an orthotopic, genetically-defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.
Publisher
Cold Spring Harbor Laboratory
Reference101 articles.
1. Trends in pancreatic adenocarcinoma incidence and mortality in the United States in the last four decades; a SEER-based study
2. American Cancer Society. Cancer Facts & Figures 2016. Atlanta: American Cancer Society; 2016. Available from: http://www.cancer.org/research/cancerfactsstatistics/cancerfactsfigures2016/index.
3. SEER. Surveillance, Epidemiology, and End Results Program Stat Fact Sheets: Pancreas Cancer. Accessed December 12, 2016. Available from: URL: http://seer.cancer.gov/statfacts/html/pancreas.html.
4. National Comprehensive Cancer Network. Pancreatic Adenocarcinoma, Version 1.2021. 23 October 2020. In: NCCN Clinical Practice Guidelines In Oncology (NCCN Guidelines®) [Internet]. Available from: https://www.nccn.org.
5. Genomic responses in mouse models poorly mimic human inflammatory diseases
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献