Abstract
Abstractthe reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed a new critical-size mandibular bone defect model on a mini-pig, close to human clinical issues. We analyzed the bone reconstruction obtained by a 3D printed scaffold made of clinical-grade PLA, coated with a polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). We compared the results (CT-scan, μCT, histology) to the gold standard solution, bone autograft. We demonstrated that the dose of BMP-2 delivered from the scaffold significantly influenced the amount of regenerated bone and the repair kinetics, with a clear BMP-2 dose-dependence. Bone was homogeneously formed inside the scaffold without ectopic bone formation. The bone repair was as good as for the bone autograft. The BMP-2 doses applied in our study were reduced 20 to 75-fold compared to the commercial collagen sponges used in the current clinical applications, without any adverse effects. 3D printed PLA scaffolds loaded with reduced doses of BMP-2 can be a safe and simple solution for large bone defects faced in the clinic.
Publisher
Cold Spring Harbor Laboratory
Reference49 articles.
1. Bone graft substitutes;Expert review of medical devices,2006
2. Bone tissue engineering: from bench to bedside;Materials Today,2012
3. S. Zeiter , K. Koschitzki , M. Alini , F. Jakob , M. Rudert , M. Herrmann , Evaluation of preclinical models for the testing of bone tissue-engineered constructs, Tissue Eng Part C Methods (2020).
4. Bone regenerative medicine: classic options, novel strategies, and future directions;Journal of orthopaedic surgery and research,2014
5. A Tissue Engineering Solution for Segmental Defect Regeneration in Load-Bearing Long Bones