Nanowires unravel a time-correlated stochastic vectorial process in cells

Abstract

AbstractA cell uses its cytoskeletal machinery to control its membrane projections to seek and obtain cargo from its microenvironment. Though this process has been studied extensively using spherical cargo, it remains largely unknown how the process operates with vectorial ones, which are non-spheroid rigid objects with an aspect ratio. In this study, a vectorial cargo, silicon nanowire, was observed to have multiple modes of initial contact and to realign along a membrane projection or on a lamella. Using a qualitative theoretical approach, we demonstrate how membrane energy fluctuations potentially drive this realignment of a vectorial cargo. This was understood by calculations which establish how aspect ratio controls the energy landscape in a vectorial object and its influence on relative energy stability of nanowire-membrane contacts. A study of the realignment transport of vectorial cargoes and their comparison with Ornstein-Uhlenbeck process simulations revealed how one-dimensional time-correlated noise manifested in the transport process. Furthermore, a comparison between sliding of nanowires on cell membrane contacts versus rotational realignment with the same model revealed identical characteristics behind both. The understanding that one-dimensional time-correlated noise underlies both sliding and rotation of a vectorial cargo establishes how cytoskeletal dynamics effectively couples their realignment with subsequent transport for phagocytosis. This work establishes the significance of vectorial cargoes and the nature of underlying vectorial processes that enable their cellular processing.