Absence-like epileptic spike-wave discharges in adult Lister Hooded rats and their pharmacological modulation

Abstract

AbstractEfforts to advance translation through pre-clinical behavioural and pharmacological tests prompted attention to rat strain differences. Particularly the use of touchscreen technology for cognitive testing initiated the widespread use of Lister Hooded and Long Evans rats and they differed in pharmacological sensitivity to certain drugs. One possible reason for this rat strain difference could be that Long Evans rats produce high-amplitude spike-wave discharges (SWDs) in their cortical EEG recordings, while no information available about Lister Hooded rats in this regard. As a serendipitous observation, we noticed the presence of SWDs during the EEG recordings of Lister Hooded rats. In this study, therefore, we examined these spontaneous SWDs in two groups of Lister Hooded rats. The number and sum duration of the SWDs were similar to that was observed in other rat strains. We found SWDs during wakefulness, slow-wave sleep (SWS) and rapid eye movement (REM) sleep, their duration was the longest during wakefulness, but their number and sum duration were also high during REM. The GABA-B receptor agonist baclofen exacerbated, while the GABA-B antagonist SCH50911 reduced the occurrence of the recorded SWDs. Typical anti-seizure medications, valproate and diazepam, decreased the number and sum duration of SWDs. Although the two rat strains typically used in touchscreen experiments are similar in term of SWDs, the occurrence and possible pharmacological modulation of SWDs are considerable during their use in behavioural experiments.