Mutation ofOsCDS5confers broad-spectrum disease resistance in rice

Abstract

AbstractPhospholipids are important components of biological membranes, participating in various biological processes, including plant development and responses to biotic and abiotic stresses. A previous study showed that mutation of the riceOsCDS5(CDP-DAGSynthase) gene alters lipid metabolism, causing enhanced abiotic stress responses, yellowing of leaves at the seedling stage and delayed plant development. Here, we observed that theOscds5mutant shows enhanced resistance to rice blast, bacterial blight and bacterial leaf streak. Mutation ofOsCDS5promotes production of reactive oxygen species (ROS) and increases the expression level of multiple defense-related genes. Transcriptomic analyses indicate that genes involved in responses to stress, biotic/abiotic stimuli and metabolic processes are highly upregulated and enriched in mutantOscds5. Metabolomic analyses show that differential metabolites are enriched in the lipid metabolic and tryptophan metabolic pathways. The decreased level of phosphatidylinositol (PI) and increased level of serotonin likely contribute to enhanced disease resistance of theOscds5mutant. Taken together, mutation ofOsCDS5enhances abiotic and biotic stress responses, andOsCDS5 may be a promising target in genetic engineering to enhance the resilience of rice to abiotic and biotic stresses simultaneously.