Competition for resources during development drives allometric patterns in the grassSetaria

Abstract

AbstractGrasses grow a series of phytomers during development. The distance between successive leaves is determined by internode lengths. Grasses exhibit genetic, developmental, and environmental variability in phytomer number, but how this affects internode length, biomass, and height is unknown. We hypothesized that a generalized mathematical model of phytomer development wherein between-phytomer competition influences internode length distributions would be sufficient to explain internode length patterns in twoSetariagenotypes: weedy A10 and domesticated B100. Our model takes a novel approach that includes the vegetative growth of leaf blade, sheath, and internode at the individual phytomer level, and the shift to reproductive growth. To validate and test our mathematical model, we carried out a greenhouse experiment. We found that the rate of leaf emergence is consistent for both genotypes across development, and that the length of time spent elongating for the leaf and internode can be described as the ratio between the time of phytomer emergence and the elongation completion time. The validated model was simulated across all possible parameter values to predict the influence of phytomer number on internode length. This analysis predicts that different internode length distributions across different numbers of total phytomers are an emergent property, rather than a genotype-specific property requiring genotype-specific models. We applied the model to internode length only field data ofS. italicaaccession B100, grown under both well-watered and drought conditions. The model predicts that droughted plants reduce leaf elongation time, reduce resource allocation to the internodes, and overall experience slower growth. Together, model and data suggest that allometric patterns are driven by competition for resources among phytomer and the shift to reproductive growth inSetaria. The resulting model enables us to predict growth dynamics and final allometries at the phytomer level.