Abstract
AbstractThis study investigates the regulation ofE. coli aphAexpression under nutrient starvation. Using transcriptional reporters with truncatedaphApromoter sequences, we found that starvation of carbon and phosphate, but not amino acid, stimulatedaphAexpression through distinct promoter regions. Deletions ofcrporcyaAreducedaphAexpression, confirming their importance inaphAregulation during carbon starvation. Conversely, CytR deletion increasedaphAexpression, suggesting CytR’s role as a repressor ofaphAexpression. Collectively, these data imply a potential connection between CytR,aphAexpression, and the broader context of natural competence evolution and bacterial nutrient absorption.
Publisher
Cold Spring Harbor Laboratory