Author:
Fu Chaoyu,Dilasser Florian,Lin Shao-Zhen,Karnat Marc,Arora Aditya,Rajendiran Harini,Ong Hui Ting,Hoon Brenda Nai Mui,Phow Sound Wai,Hirashima Tsuyoshi,Sheetz Michael,Rupprecht Jean-François,Tlili Sham,Viasnoff Virgile
Abstract
AbstractActomyosin tension has been shown to be a ubiquitous driver of tissue morphogenesis1, 2. The Rho pathway, a prominent regulatory network influencing cortical tension, plays a central role in both tissue reorganisation and cell migration3–6. While viscous dissipation in the actin network is commonly regarded as a constant passive parameter in cell migration in both 2D and 3D contexts, there is limited knowledge concerning the regulation of dissipative forces arising from viscous drag between cells during collective rearrangement. Here, we found that the phosphorylation of Epithelial Growth Factor Receptor (EGFR) downstream ofde novoE-cadherin adhesion7, 8orchestrates a feedback loop, thereby governing intercellular viscosity via the Rac pathway regulating actin dynamics. Our findings highlight how the E-cadherin-dependent EGFR activity controls the migration mode of collective cell movements independently of intercellular tension. Combining molecular cell biology, micropatterning, andin silicosimulation, our work suggests the existence of a regulatory loop by which cells can tune junctional actin viscosity, with implications for the phenomenology of morphogenetic movements.
Publisher
Cold Spring Harbor Laboratory