Integrated bioinformatics and wet-lab analysis revealed prominent inflammatory genes of Extracellular Matrix as prognostic biomarkers in patients with advance IBD requiring early surgery

Abstract

AbstractBackgroundThe number of patients with inflammatory bowel disease (IBD) is increasing worldwide. Due to the fact that at the age of 20 to 30 years, this autoimmune disease is very common; Investigating and identifying prognostic biomarkers in advanced IBD is very important; Because according to the identification of these biomarkers, patients who need early surgery can be nominated and undergo surgery without wasting time and treatment costs. In this study, with the aim of identifying effective biomarkers involved in the inflammatory part of extracellular matrix (ECM) in the early surgery of IBD, separately from Crohns disease and ulcerative colitis.MethodIn this study, we examined 50 patients in both patient groups as well as the normal group. The expression of the nominated genes MASP2, DKC1, HNF4A, and STAT3 was analyzed using quantitative polymerase chain reaction (Q-PCR) and relative quantification was determined using the 2-ΔΔCtmethod. ROC curve analysis was performed to compare IBD (UC & CD) and normal for the investigated genes. The correlation between adhesion molecule gene expression and immunophenotype was analyzed. Also we comprehensively analyzed the genetic alteration, prognostic value and gene regulatory networks using multiple databases.ResultThe obtained results showed that MASP2 and DKC1 genes were significantly expressed in advanced UC patients, as well as HNF4A and STAT3 in advanced CD patients.ConclusionIt can be stated that the biomarker panel MASP2, DKC1, HNF4A, and STAT3 related to them have a significant prognostic role in the candidates of IBD patients for early surgery.Graphical abstract