First animal source metagenome assembly ofLawsonella clevelandensisfrom canine external otitis

Abstract

ABSTRACTExternal otitis is one of the most common conditions in dogs to be presented to the veterinarian. Moreover, the disorder is often difficult to manage. The range and role of microorganisms involved in the pathogenesis are currently not fully understood. Therefore, the condition has been studied using third-generation sequencing (Oxford Nanopore Technology) to gain a more complete picture of the pathogens involved. Throughout the metagenome assembly of a sample harvested from the ear canal of an 11-year-old female Yorkshire terrier suffering from chronic external otitis, a genome ofLawsonella clevelandensiswas compiled. To our knowledge, this result is the first of its type of animal origin. The outcome of the assembly (CP140010) is a single circular chromosome with a length of 1,878,509 bp, and 1,826 predicted protein-coding genes. No open reading frames associated with antimicrobial resistance could have been identified.