Author:
Edwards-Ingram Laura C.,Gent Manda E.,Hoyle David C.,Hayes Andrew,Stateva Lubomira I.,Oliver Stephen G.
Abstract
The science of taxonomy is constantly improving as new techniques are developed. Current practice is to construct phylogenetic trees based on the analysis of the DNA sequence of single genes, or parts of single genes. However, this approach has recently been brought into question as several tree topologies may be produced for the same clade when the sequences for various different genes are used. The availability of complete genome sequences for several organisms has seen the adoption of microarray technology to construct molecular phylogenies of bacteria, based on all of the genes. Similar techniques have been used to reveal the relationships between different strains of the yeast Saccharomyces cerevisiae. We have exploited microarray technology to construct a molecular phylogeny for the Saccharomyces sensu stricto complex of yeast species, which is based on all of the protein-encoding genes revealed by the complete genome sequence of the paradigmatic species, S. cerevisiae. We also analyze different strains of S. cerevisiae itself, as well as the putative species S. boulardii. We show that in addition to the phylogeny produced, we can identify and analyze individual ORF traits and interpret the results to give a detailed explanation of evolutionary events underlying the phylogeny.
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics(clinical),Genetics
Reference62 articles.
1. Adams, A., Gottschling, D.E., Kaiser, C.A., and Stearns, T. 1997. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Yeast: an Experimental Organism for Modern Biology
3. Brown, T.A. 2002. Genomes. BIOS Scientific Publishers, New York.
4. Finding Functional Features in
Saccharomyces
Genomes by Phylogenetic Footprinting
5. Chromosomal polymorphism and adaptation to specific industrial environments of Saccharomyces strains
Cited by
65 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献