Author:
Petersen Kenneth H,Lohse Jesper,Ramsgaard Lasse
Abstract
AbstractAutomated IHC double staining using DAB and HRP Magenta is illustrated utilizing a new acid block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
Publisher
Cold Spring Harbor Laboratory