Physiology and lipid accumulation capacity of differentYarrowia lipolyticaandRhodosporidium toruloidesstrains on glycerol

Abstract

AbstractObjectiveTo compare physiological and process parameters, as well as lipid accumulation capacity, of six strains ofYarrowia lipolyticaand two strains ofRhodosporidium toruloidesin media containing glycerol as the main carbon and energy source.ResultsThe strainsY. lipolyticaIMUFRJ 50678, Po1g, W29 and CCT 5443 displayed very similar physiological parameters, with µmax,andaverage values of 0.31 h−1, 0.53 (g DW/g S), 0.60 (g S/g DW.h) and 0.46 (g DW/g S), respectively. The two strains ofR. toruloidespresented physiological and process parameters with no significant difference, with average values of 0.084 h−1, 0.53 (g DW/g S), 0.17 (g S/g DW.h) and 0.44 (g DW/g S). Among allY. lipolyticastrains,Y. lipolyticaCCT 5443 strain presented the highestYLip/S, 0.054 (g Lip/g S), andPLipof 0.040 (g Lip/l.h). Among all investigated strains (Y. lipolyticaandR. toruloides), the yeastR. toruloidesCCT 7815 displayed the highest lipid accumulation capacity, withYLip/Sequal to 0.11 (g Lip/g S) andPLipequal to 0.10 (g Lip/l.h).ConclusionAmong all strains investigated in our study, the yeast strainR. toruloidesCCT 7815 presents the most promising characteristics for industrial single cell oil production.List of abbreviationsDWdry weight (g/l)Liplipid (g/l)PLiplipid productivity (g Lip/l.h)maximum specific substrate consumption rate (g S/g DW.h)Ssubstrate (g/l)YLip/Slipid yield on substrate (g Lip/g S)biomass yield on substrate during the exponential growth phase (g DW/g S)global biomass yield on substrate (g DW/g S)µmaxmaximum specific growth rate (h−1)