Author:
Amat Ramon,Böttcher René,Le Dily François,Vidal Enrique,Quilez Javier,Cuartero Yasmina,Beato Miguel,de Nadal Eulàlia,Posas Francesc
Abstract
Nuclear architecture is decisive for the assembly of transcriptional responses. However, how chromosome organization is dynamically modulated to permit rapid and transient transcriptional changes in response to environmental challenges remains unclear. Here we show that hyperosmotic stress disrupts different levels of chromosome organization, ranging from A/B compartment changes to reduction in the number and insulation of topologically associating domains (TADs). Concomitantly, transcription is greatly affected, TAD borders weaken, and RNA Polymerase II runs off from hundreds of transcription end sites. Stress alters the binding profiles of architectural proteins, which explains the disappearance of local chromatin organization. These processes are dynamic, and cells rapidly reconstitute their default chromatin conformation after stress removal, uncovering an intrinsic organization. Transcription is not required for local chromatin reorganization, while compartment recovery is partially transcription-dependent. Thus, nuclear organization in mammalian cells can be rapidly modulated by environmental changes in a reversible manner.
Funder
Beatriu de Pinós
Marie-Curie Cofund program fellowship
Juan de la Cierva fellowship
Spanish Ministry of Economy and Competitiveness
Catalan Government
Fundación Botín
Banco Santander
Unidad de Excelencia Maria de Maeztu
ICREA Acadèmia
European Research Council
CERCA
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics (clinical),Genetics
Cited by
36 articles.
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