Patterns of resistance to antibiotics of Enterococcus faecalis in clinical isolates of diabetic foot and presence of culture resistant to linezolid

Abstract

AbstractIntroductionHigh levels of hyperglycemia lead to diabetes, the same levels that if not controlled increase the risk of diabetic neuropathy, which over time translates into loss of sensation and lesions that progress and lead to diabetic foot may occur, microorganisms Mainly Gram positive cocci and Gram negative bacilli, as well as yeasts are those that are mainly found in this metabolic and infectious pathology.MethodsDiabetic foot wounds were studied in patients aged 45-54 years, with a mean age of 50 years, who had different periods of time with a hyperglycemia problem and different complications associated with diabetic foot, and were not considered as exclusion criteria. With a total of 41 cultures, 25 belong to male patients and 16 to female patients. All of these in the Toluca Valley, Mexico. The primary swab was reseeded in chromogenic agar, BHI, Salt and mannitol, calf blood, EMB and MacConkey, after 6 hours after taking the sample. In addition to microbial reseeding, a Gram stain was performed on each of the samples. The Petri dishes were placed in an incubation oven for 18 hours at 35 ° C with ± 2 ° C. Bacterial identification was performed on automated equipment from Beckman Coulter, as well as antibiotic sensitivity tests.Results35 positive cultures were obtained, of which they had a single microbial agent and some cases with two agents, which could be bacteria-bacteria or bacteria-yeast. With 14 positive strains for Enterococcus faecalis, with 100% sensitivity for cell wall inhibitors, and high resistance to tetracycline with 85.71% and 92.86% resistance to the macrolide erythromycin. In addition, there is a strain that was resistant to linezolid, and variable resistance patterns in fluoroquinolones and other antibiotics.