Author:
Bergamelli Mathilde,Martin Hélène,Bénard Mélinda,Ausseil Jérôme,Mansuy Jean-Michel,Hurbain Ilse,Mouysset Maïlys,Groussolles Marion,Cartron Géraldine,Gac Yann Tanguy le,Moinard Nathalie,Suberbielle Elsa,Izopet Jacques,Tscherning Charlotte,Raposo Graça,Gonzalez-Dunia Daniel,D’Angelo Gisela,Malnou Cécile E.
Abstract
ABSTRACTCurrently, research on the use of non-invasive biomarkers as diagnosis and prognosis tools during pathological pregnancies is in full development. Among these, placenta-derived small extracellular vesicles (sEVs) are considered as serious candidates, since their composition is modified during many pregnancy pathologies. Moreover, sEVs are found in maternal serum and can thus be easily purified from a simple blood sample. In this study, we describe the isolation of sEVs from a histoculture model of first trimester placental explants. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these sEVs and defined their associated markers and ultrastructure. We next examined the consequences of infection by human cytomegalovirus on sEVs secretion and characteristics. We observed that infection led to increased levels of expression of several surface markers, without any impact on the secretion and integrity of sEVs. Our findings open the prospect for the identification of new predictive biomarkers for the severity and outcome of this congenital infection early during pregnancy, which are still sorely lacking.
Publisher
Cold Spring Harbor Laboratory