The distinct roles of calcium in rapid control of neuronal glycolysis and the tricarboxylic acid cycle

Abstract

ABSTRACTWhen neurons engage in intense periods of activity, the consequent increase in energy demand can be counteracted by the coordinated activation of glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation. However, the trigger for glycolytic activation is unknown and the role for Ca2+in the mitochondrial responses has been debated. Using genetically-encoded fluorescent biosensors and NAD(P)H autofluorescence imaging in acute hippocampal slices, here we find that Ca2+uptake into the mitochondria is responsible for the buildup of mitochondrial NADH, probably through Ca2+activation of dehydrogenases in the TCA cycle. In the cytosol, we do not observe a role for the Ca2+/calmodulin signaling pathway, or AMPK, in mediating the rise in glycolytic NADH in response to acute stimulation. Calcium, nevertheless, is a major contributor to glycolysis, although not strictly necessary. Aerobic glycolysis in neurons is triggered mainly by the energy demand resulting from either Na+or Ca2+extrusion.Impact StatementWhen neurons are stimulated, calcium influx instructs mitochondria to increase energy metabolism, but it is increased energy demand in cytosol rather than Ca2+signaling that leads to increased neuronal glycolysis.