Author:
Zhang Zhimin,Huang Yuran,Tao Wenli,Wei Yunfei,Xu Liang,Gong Wenwen,Zhang Yuhui,Zhou Jiaqiang,Cao Liangcai,Liu Yong,Han Yubing,Kuang Cuifang,Liu Xu
Abstract
AbstractStimulated emission depletion microscopy (STED) is a powerful tool for studying nanoscale cell structure and activity, but the difficulties it encounters in multicolor imaging limit its application in biological research. To overcome the disadvantages of limited number of channels and high cost of multicolor STED imaging based on spectral identity, we introduced lifetime into live-cell multicolor STED imaging by separating selected dyes of the same spectrum by phasor analysis. Experimental results show that our method enables live-cell STED imaging with at least 4 colors, enabling observation of cellular activity beyond the diffraction limit.
Publisher
Cold Spring Harbor Laboratory