Influence of Diabetes Mellitus Type 2 on the angiogenic potential of Bone Marrow derived Mesenchymal Stem Cells

Abstract

ABSTRACTMesenchymal stem cells (MSCs) are used as novel therapeutic tools in cell-based therapies including diabetic complications. However, diabetes may negatively affect the therapeutic potential of autologous MSCs. In order to enhance the utilization of diabetic MSCs, better characterization of their angiogenic capacity should be performed. The aim of this study was to investigate the effect of diabetes mellitus (DM) on the angiogenic potential of bone marrow derived MSCs. MSCs were isolated from DM subjects and were compared with MSCs isolated from non-DM subjects. The angiogenic potential of MSCs was assessed in which there was insignificant difference in the proliferation and expression of angiogenic factors between the two groups. Moreover, no statistically significant change was found in the viability and angiogenic activities of endothelial cells isolated from both groups. Results indicate that the hyperglycemic milieu had no significant impact on the angiogenic-related functional properties of MSCs and they are able to survive the harsh diabetic conditions.