Bifunctional Monosaccharides Preferentially Localize to Nuclear Subcompartments

Abstract

AbstractRecent progress in glycan research has been driven by widespread implementations of metabolic oligosaccharide engineering. Complementing existing approaches, we here introduce bifunctional, UV-crosslinkable and clickable N-acetylglucosamine and N-acetylgalactosamine analogues, which enable direct visualization of the intracellular probe distribution as well as distinguishing monomeric and macromolecule-bound fractions. Using this feature, we find that monomeric N-acetylmonosaccharides partition into RNA-rich nuclear compartments such as nuclear speckles and nucleoli. This suggests the existence of spatially separated N-acetylmonosccharide pools within the nucleoplasm. Taken together, bifunctional N-acetylmonosaccharide probes are a powerful discovery tool for probing intracellular localization of monosaccharides and glycosylated macromolecules.