Genome-based metabolic and phylogenomic analysis of the type strains ofTerrisporobactermayombei,Terrisporobacter petroleariusandTerrisporobacter glycolicus

Abstract

AbstractTo date, three validly published type strains of the genusTerrisporobacterare covered by draft genome sequences, and the genes and pathway responsible for acetogenesis have not been analyzed. Here, we report complete genome sequences of the bacterial type strainsTerrisporobacter petroleariusJCM 19845T,Terrisporobacter mayombeiDSM 6539TandTerrisporobacter glycolicusDSM 1288T. Functional annotation, KEGG pathway module reconstructions and screening for virulence factors were performed. Various species-specific vitamin, cofactor and amino acid auxotrophies were identified and a model for acetogenesis ofTerrisporobacterwas constructed. The complete genomes harbored a gene cluster for the reductive proline-dependent branch of the Stickland reaction located on an approximately 21 kb plasmid, which is exclusively found in theTerrisporobactergenus. Phylogenomic analysis of availableTerrisporobactergenomes suggested a reclassification of most isolates asT. glycolicusintoT. petrolearius.