Author:
Lian Tengfei,Guan Ruifang,Zhou Bing-Rui,Bai Yawen
Abstract
AbstractPioneer transcription factors are vital for cell fate changes. PU.1 and C/EBP⍺ work together to regulate hematopoietic stem cell differentiation. However, how they recognizein vivonucleosomal DNA targets remain elusive. Here we report the structures of the nucleosome containing the mouse genomicCX3CR1enhancer DNA and its complexes with PU.1 alone and with both PU.1 and the C/EBP⍺ DNA binding domain. Our structures reveal that PU.1 binds the DNA motif at the exit linker, shifting 17 bp of DNA into the core region through interactions with H2A, unwrapping ∼20 bp of nucleosomal DNA. C/EBP⍺ binding, aided by PU.1’s repositioning, unwraps ∼25 bp entry DNA. The PU.1 Q218H mutation, linked to acute myeloid leukemia, disrupts PU.1-H2A interactions. PU.1 and C/EBP⍺ jointly displace linker histone H1 and open the H1-condensed nucleosome array. Our study unveils how two pioneer factors can work cooperatively to open closed chromatin by altering DNA positioning in the nucleosome.
Publisher
Cold Spring Harbor Laboratory