Characterization of the mitochondrial proteome in the ctenophoreMnemiopsis leidyiusing MitoPredictor

Abstract

ii.AbstractMitochondrial proteomes have been experimentally characterized for only a handful of animal species. However, the increasing availability of genomic and transcriptomic data allows one to infer mitochondrial proteins using computational tools. MitoPredictor is a novel random-forest classifier, which utilizes orthology-search, mitochondrial targeting signal (MTS) identification, and protein domain content to infer mitochondrial proteins in animals. MitoPredictor’s output also includes an easy-to-use R Shiny applet for the visualization and analysis of the results. In this article, we provide a guide for predicting and analyzing the mitochondrial proteome of the ctenophoreMnemiopsis leidyiusing MitoPredictor.