Abstract
AbstractMolecular tools for modulating transgene expression inAedes aegyptiare few. Here we demonstrate that adjustments to theAePUbpromoter length can alter expression levels of two reporter proteins inAe. aegypticell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
Publisher
Cold Spring Harbor Laboratory
Reference21 articles.
1. Viglietta M , Bellone R , Blisnick AA , Failloux AB . Vector Specificity of Arbovirus Transmission. Front Microbiol [Internet]. 2021 [cited 2023 Jun 17];12. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2021.773211
2. Expanding the CRISPR Toolbox in Culicine Mosquitoes: In Vitro Validation of Pol III Promoters;ACS Synth Biol,2020
3. A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines;Sci Rep,2021
4. Green fluorescent protein as a genetic marker in transgenic Aedes aegypti
5. Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines;Gene,1997
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献