Abstract
ABSTRACTThe continuous spread of highly transmissible variants of concern and the potential diminished effectiveness of existing vaccines necessitate ongoing research and development of new vaccines. Immunogenic molecule-anchored antigen has demonstrated superior efficacy in subunit vaccination, primarily due to enhanced cellular uptake facilitated by the affinity between the surface of Immunogenic molecule and the cell membrane. Based on the Immunogenic recombinaseB. malayiRecA (BmRecA), we have overexpressed the construct ofBmRecA with SARS-CoV-2 RBD (BmRecA-RBD) that exists as a stable helical filament formation; it was purified and crystallized to obtain X-ray diffraction data at 2.7 Å, belonged to the hexagonal symmetry groupP65in the unit-cell parameters of a=b=122.12, c=75.55 and α=β=90°, γ=120°. The Matthews coefficient was estimated to be 3.12 Å3Da-1, corresponding to solvent contents of 52.65.
Publisher
Cold Spring Harbor Laboratory
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