Author:
Honari Maryam,Ashnest Joanne R.,Sharbel Timothy F.
Abstract
AbstractAmong candidate genes underlying the control components of apomixis, APOLLO is known for its strong linkage to apomeiosis in the genusBoechera. The gene has “apo-alleles”, which are characterized by a set of linked apomixis-specific polymorphisms, and “sex-alleles”. All apomictic□Boecheragenotypes are heterozygous for the apo/sex-alleles, while all sexual genotypes are homozygous for sex-alleles. In this study, native and synthetic APOLLO promoters were characterized by detecting the expression level of the β-glucuronidase (GUS) gene inArabidopsis. Comparing various flower developmental stages in transgenic lines containing different constructs with 2 kb native transgenic lines revealed that changes to the APOLLO promoter causes shifts in tissue- and developmental-stage specificity of GUS expression. Importantly, several apomixis-specific polymorphisms in the 5’UTR change the timing and location of GUS activity from somatic to reproductive tissues. These synthetic data simulate a plausible evolutionary process whereby apomixis-specific gene activity can be achieved.
Publisher
Cold Spring Harbor Laboratory