phospholipase C gamma-1is required downstream ofvascular endothelial growth factorduring arterial development

Abstract

In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find thaty10encodesphospholipase C gamma-1(plcg1), a known effector of receptor tyrosine kinase signaling. We further show thatplcg1y10mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.