Abstract
SummaryPolycomb silencing represses gene expression and provides a molecular memory of chromatin state that is essential for animal development. We show thatDrosophilafemale germline stem cells (GSCs) provide a powerful system for studying Polycomb silencing and how it is established. GSCs resemble pluripotent mammalian embryonic cells in lacking silenced chromatin, but most GSC daughters, like typical somatic cells, induce Polycomb silencing as they differentiate into nurse cells. Developmentally controlled changes in the levels of two Polycomb repressive complex 2 (PRC2)-interacting proteins, Pcl and Scm, initiate differentiation. In germline stem cells, abundant Pcl inhibits silencing by slowing PRC2 and diverting it from PRE sequences. During differentiation, core PRC2 represses inactive loci while Scm and residual Pcl cooperate to enrich PRC2 and silence traditional Polycomb domains. We propose that PRC2-interacting proteins regulate the transition from a variable to stable transcription state during differentiation by altering the rate that PRC2 samples regulatory sequences.
Publisher
Cold Spring Harbor Laboratory
Reference131 articles.
1. Alhaj Abed, J. , Ghotbi, E. , Ye, P. , Frolov, A. , Benes, J. , and Jones, R.S. (2018). De novo recruitment of Polycomb-group proteins in Drosophila embryos. Development 145, dev165027.
2. Polycomb complexes in stem cells and embryonic development
3. Paucity and preferential suppression of transgenes in late replication domains of the D. melanogaster genome
4. Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation
5. Blackledge, N.P. , Fursova, N.A. , Kelley, J.R. , Huseyin, M.K. , Feldmann, A. , and Klose, R.J . (2019). PRC1 Catalytic Activity Is Central to Polycomb System Function. Mol. Cell.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献